Why is bsa used in restriction digest

How do I perform a double digest with an enzyme that comes with the new buffer and an enzyme that comes with the old buffer system? We recommend using the Double Digest Finder and use the buffer it recommends. Alternatively, if it recommends CutSmart Buffer, then you can simply use the new buffer. My restriction enzyme used to be available at a lower concentration. This contributes to ease of use and often results in improved performance by having less glycerol in the digest.

Why did you add BSA into all the restriction enzyme reaction buffers? As an added convenience for our customers, NEB has included BSA in all its restriction enzyme buffers, eliminating the need to add it as a separate step. This makes digestion reactions simpler, set-up time even faster, and decreases the number of tubes to store.

Why did you remove DTT from your restriction enzyme buffers? DTT was originally included in the buffer formulation for historical reasons. The source of purified BSA is a molecular biology grade, protease free, fraction V starting material. This material is then processed to remove nucleases.

This assay checks for endonuclease, exonuclease and binding protein contamination. Produced in ISO-certificated Company. Transport: Ambient or with blue ice Note: - It is do not recommend using this preparation of BSA as a standard for quantitation purposes or size determination.

At this level the buffer from the BSA have a very small effect, only. Either email addresses are anonymous for this group or you need the view member email addresses permission to view the original message. We London biohackspace were planning to do a restriction digest on wednesday. It would be using Kpn1 on a PCR product for viewing on a gel. No further processing required. Having just looked at the info sheet for Kpn1 from Sigma I noticed that the enzyme buffer solution requires the addition of BSA.

We haven't used restriction enzymes before; is it worth leaving out the BSA or should we wait to get some before we proceed? It is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels.

This protein does not affect other enzymes that do not need it for stabilization.